Phosphorothioate oligonucleotides separation in ion-pairing reversed-phase liquid chromatography: Effect of ion-pairing system

Journal of Chromatography A(2022)

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摘要
•IP amines effect on retention and diastereomeric separation of PS ONs was evaluated.•Stronger IP systems suppress diastereomer separation more effectively.•More hydrophobic IP systems provide baseline resolution of n and n-1 mers.•Highly hydrophobic and concentrated IP system is not suitable for ON analysis.•Separation of ONs with various number/position of PS modifications was achieved.
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关键词
Phosphorothioate oligonucleotides,Ion-pairing reversed-phase chromatography,Diastereomeric separation,Ion-pairing agent,Resolution
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