Mp37-03 extracorporeal shockwave therapy improves cp/cpps by down-regulating nlpr3 inflammasome in a prostatitis rat model

You have accessJournal of UrologyCME1 May 2022MP37-03 EXTRACORPOREAL SHOCKWAVE THERAPY IMPROVES CP/CPPS BY DOWN-REGULATING NLPR3 INFLAMMASOME IN A PROSTATITIS RAT MODEL Dongho Shin, Chang Eil Yoon, Hyeok Jae Kwon, Hyong Woo Moon, Yong Hyun Park, Hyuk Jin Cho, U-syn Ha, Sung-Hoo Hong, Sae Woong Kim, Ji Youl Lee, and Woong Jin Bae Dongho ShinDongho Shin More articles by this author , Chang Eil YoonChang Eil Yoon More articles by this author , Hyeok Jae KwonHyeok Jae Kwon More articles by this author , Hyong Woo MoonHyong Woo Moon More articles by this author , Yong Hyun ParkYong Hyun Park More articles by this author , Hyuk Jin ChoHyuk Jin Cho More articles by this author , U-syn HaU-syn Ha More articles by this author , Sung-Hoo HongSung-Hoo Hong More articles by this author , Sae Woong KimSae Woong Kim More articles by this author , Ji Youl LeeJi Youl Lee More articles by this author , and Woong Jin BaeWoong Jin Bae More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002591.03AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: The aim of this study was to evaluate the anti-inflammatory and antioxidative effects of extracorporeal shockwave therapy (ESWT) on prostatitis using in-vitro as well as in-vivo experimental models and explore the mechanism. METHODS: RWPE-1 cells randomly divided into 5 groups: 1 RWPE-1 group (normal control), 2 LPS group (lipopolysaccharide inducing inflammation), 3 0.1ESWT group (treated by 0.1mJ/mm2 ESWT), 4 0.2ESWT group (treated by 0.2mJ/mm2 ESWT) and 5 0.3ESWT group (treated by 0.3mJ/mm2 ESWT). After ESWT administered, cells and supernatant were collected for ELISA and western blot. In vivo, Sprague-Dawley male rats (n=36) were randomly divided into 3 groups: 1 normal group, 2 prostatitis group, and 3 ESWT group (n=12 for each). Prostatitis was induced by 17 beta-estradiol and dihydrotestosterone(DHT) administration. Four weeks after ESWT, pain index was assessed for all groups and prostate tissues were collected for immunohistochemistry, immunofluorescence, apoptosis analysis and Western blot. RESULTS: The optimal energy flux density of ESWT was 0.2 mJ/mm2 in vitro. ESWT ameliorated discomfort in rats with prostatitis. Inflammation was improved by ESWT in rats with prostatitis (P<0.05). Compared to normal rats, overexpressed NLRP3 inflammasomes triggered apoptosis which was improved by ESWT in prostatitis (P<0.05). TLR4-NFκB pathway was overactive with prostatitis, compared to normal and ESWT group (P<0.05). And BAX/BAK pathway was inhibited by ESWT in prostatitis rats (P<0.05). CONCLUSIONS: ESWT improved CP/CPPS by reducing NLPR3 inflammasome and ameliorated apoptosis via inhibiting BAX/BAK pathway in a rat model. TLR4 might be the key protein bonding NLPR3 inflammasome and BAX/BAK pathway. ESWT should be a potential and promising approach for the treatment for CP/CPPS. Source of Funding: none © 2022 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 207Issue Supplement 5May 2022Page: e608 Advertisement Copyright & Permissions© 2022 by American Urological Association Education and Research, Inc.MetricsAuthor Information Dongho Shin More articles by this author Chang Eil Yoon More articles by this author Hyeok Jae Kwon More articles by this author Hyong Woo Moon More articles by this author Yong Hyun Park More articles by this author Hyuk Jin Cho More articles by this author U-syn Ha More articles by this author Sung-Hoo Hong More articles by this author Sae Woong Kim More articles by this author Ji Youl Lee More articles by this author Woong Jin Bae More articles by this author Expand All Advertisement PDF DownloadLoading ...