Influence of media and cellular origin on growth factors secreted by mesenchymal stem cells

Thi Xuan Phuong Do,Thi Ngoc Ha Nguyen, Thi Hien Mai, Huong Diem Hoang,Thi My Nhung Hoang, Thanh Liem Nguyen,Thi Trang Uyen Than

Tạp chí Sinh lý học Việt Nam(2021)

引用 0|浏览3
暂无评分
摘要
Objective: Investigating influences of the culture media and cellular origin on the expression of several growth factors carried by exosomes secreted from umbilical cord-derived mesenchymal stem cells (UCMSC) and bone marrow-derived mesenchymal stem cells (BMMSC). Methods: 03 UCMSC samples was cultured in StemMACs and PowerStem, 03 BMMSC samples was cultured in StemMAC sand ACF Plus. At the passage 2, conditioned media were collected for exosome isolation. Exosomes were then investigated for the expression of growth factors, including: vascular endothelial growth factor A (VEGF-A), fibroblast growth factor 2(FGF-2), hepatocellular growth factor (HGF) and platelet-derived growth factor BB(PDGF-BB). Results: Both UCMSC and BMMSC at secreted exosomes carrying growth factors with different amount. UCMSC cultured in PowerStem could secret exosomes with the largest amount of growth factors. Besides, UCMSC and BMMSC cultured in StemMACs secreted exosomes carrying fewer amounts of growth factors. Secretion of growth factors through exosomes depends on the cell origin where both cell types were cultured in StemMACs, only UCMSC secretedFGF-2 and PDGF-BB but BMMSC did not. Conclusion: Both UCMSC and BMMSC cultured in commercial serum- and xeno-free media could secret exosomes into cell culture media. These exosomes carried growth factors, including VEGF-A, FGF-2, HGF and PDGF-BB, with different expression levels.
更多
查看译文
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要