Anti-colorectal Cancer Effect of Ganjang Prepared with Washed-Dehydrated Solar Salt on C57BL/6 Mice

This study evaluated the anticancer effects of Ganjang (Korean soysauce) prepared with Korean solar salt without bittern (washed-dehydrated solar salt), on the azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colon cancer in C57BL/6 mice.Ganjang samples were prepared using different salts, including purified salt (PSG), generally prepared solar salt (GSG), washed and dehydrated solar salt (WDG), and washed, dehydrated, and dried solar salt (WDDG).Compared to other samples, the physico-chemical characteristics of WDG showed lower pH, higher acidity and better amino and ammonia type nitrogen content.WDG and GSG resulted in increased mouse body weight as compared to Con 1 (AOM/DSS), Con 2 (AOM/DSS＋18% purified salt water) and aPSG (AOM/DSS＋purified salt Ganjang) after 7 weeks.aWDG (AOM/DSS＋washed-dehydrated solar salt Ganjang) and aWDDG (AOM/DSS＋ washed-dehydrated-dried solar salt Ganjang) treatment significantly suppress colon length shortening and tumor counts in mouse colon, as compared to Con 1, Con 2, and aPSG (P<0.05).mRNA expressions of apoptosis related factors (Bax, in colon tissues of mice were also regulated after aWDG and aWDDG exposure.These results indicate that solar salts and different processing methods of the solar salts induces increased anti-cancer effects of Ganjang.