Genetically Encoded Aryl Alkyne for Raman Spectral Imaging of Intracellular α-Synuclein Fibrils

•Detection of amyloids in cells requires unique spectral signatures.•The aryl alkyne FCC is a site-specific conformational probe of α-syn aggregation.•Cellular Raman mapping uniquely identifies internalized FCC-labeled α-syn fibrils.•Spectral analysis reveals differences in lipid and protein recruitment to fibrils.•Raman spectral imaging is a powerful approach to study amyloid structure in cells.