Bio-Environmental preparation of Selenium Nanoparticle using Klebsiella Pneumonia and their Biomedical Activity

Abstract The aim of this study was to study the biomedical activity (biofilm by fluoresence microscope) and Characterization of Selenium NPs synthesized by klebsiella pneumonia. Different types of bacterial strains (S1-S35) aureus, were screened for biosynthesis of selenium NPs. Isolate S6 was selected as efficient isolate based on color change and antibacterial activity against the pathogenic bacteria, and it was diagnosed as klebsiella pneumonia depending on the morphology, microscopic examination and VITEK2 compact system. Characterization of bio-selenium NPs was performed using: UV-visible spectroscopy was used to characterize the synthesis of selenium NPs by Klebsiella pneumonia, with an absorption peak at 280 nm. The size and structure of the bioavailable selenium NPs were measured using an XRD occurring at 20°-26°. AFM analysis showed that the mean diameter of the bio-selenium NPs was 47. 18. Antibacterial activities have been studied for bio-selenium against pathogenic bacteria (B. cepacia, S. aureus, A. baumanni and E. coli).Biogenic Selenium expressed high significant antibiofilm activity with increasing concentration of Selenium NPs. DPPH reducing activity of nanoparticles which increased with increase concentration of biogenic Selenium NPs.