Kinetic study of complicated anti-solvent and cooling crystallization of disodium 5'-ribonucleotide

Disodium 5'-ribonucleotide, which is composed of disodium 5'-inosine (IMP) and disodium 5'-guanosine (GMP), is an important food additive. The lack of kinetic studies of it causes a lack of clarity in understanding the complicated multi-solute crystallization of IMP + GMP in ethanol-water. In this work, process analytical technology tools were used to obtain the thermodynamics and kinetic data from the experiments, the kinetic parameters of anti-solvent and cooling crystallization were investigated. The crystal form of IMP + GMP mixed crystal was determined, which was consistent with the IMP whether crystallized from pure water or ethanol-water. The effects of different anti-solvent addition rates and cooling rates on the metastable zone widths were studied, and the opposite effect on metastable zone width was found. The modified exponential empirical function was developed to correlate nucleation and growth kinetic equations under different conditions. The kinetic data were well fitted with adjusted correlation coefficient (adj-R2 > 0.7), which is sufficient to provide a valid reference for process design and control. (c) 2022 Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.