Effect of the hydroalcoholic extract of the Lampaya medicinalis Phil. on fatty acid-induced inflammatory markers in human hepatocytes HepG2

Introduction: Nonalcoholic fatty liver disease (NAFLD) is characterized by the accumulation of lipid droplets (LD) and overexpression of the LO-associated protein Perilipin 1 (PLIN1). NF-kappa B, caspase-1 and proinflammatory cytokine such as IL-1 beta participate in the pathogenesis and progression of NAFLD. Traditional medicine in northern Chile uses the plant Lampaya medicinalis Phil. (Verbenaceae) against diseases. Objective: To evaluate the effect of a hydroalcoholic extract of lampaya (HEL) on the expression of inflammatory markers and LD-associated proteins in hepatocytes treated with fatty acids. Methods: HepG2 hepatocytes were incubated with 0.66 mM oleic acid (OA) and 0.33 mM palmitic acid (PA) for 24 or 48 h in the presence or not of HEL. The protein expression of NF-KB, PLIN1 and caspase-1 was evaluated by Western blot while the mRNA expression of IL-1 beta was assessed by qPCR. Results: hepatocytes treated for 48 h with OA/AP showed an increase in IL-1 beta expression that was reversed by co-incubation with HEL. Conclusion: These antecedents provide new evidence regarding the biological activity of HEL in a model of metabolic and inflammatory alterations, associated with NAFLD, induced by OA/PA in human hepatocytes.