Cytotoxic compounds from Goniothalamus repevensis Pierre ex Finet & Gagnep

Phytochemical investigation of the methanol extract of the leaves and twigs of Goniothalamus repevensis led to the isolation of five known compounds including two styryl lactones; (-)-goniodiol-7-monoacetate (1) and (-)-goniodiol diacetate (2) as well as three aristolactam alkaloids; piperolactam C (3), aristolactam A III (4), and aristolactam B III (5). The structure determination was established by various spectroscopic methods and by comparison with the literature data. Cytotoxic activity against a panel of mammalian cancerous cell lines of compounds 1-4 was investigated. Piperolactam C (3) was found to be active in all tested cell lines with IC50 values in the range of 3.30-64.6 mu M. (-)-Goniodiol-7-monoacetate (1) exhibited cytotoxic activity only against ASK cells with IC50 value of 10.28 mu M. Having been isolated as a major component, compound 1 can be considered chemotaxonomic significance for G. repevensis.