Identification of Key MicroRNAs Regulating ELOVL6 and Glioblastoma Tumorigenesis

ELOVL fatty acid elongase 6 (ELOVL6) controls cellular fatty acid (FA) composition by catalyzing the elongation of palmitate (C16:0) to stearate (C18:0) and palmitoleate (C16:1n-7) to vaccinate (C18:1n-7). Although the transcriptional regulation of has been well studied, the post-transcriptional regulation of is not fully understood. Therefore, this study aims to evaluate the role of microRNAs (miRNAs) in regulating human . Bioinformatic analysis identified five putative miRNAs: miR-135b-5p, miR-135a-5p, miR-125a-5p, miR-125b-5p, and miR-22-3p, which potentially bind 3'-untranslated region (UTR). Results from dual-luciferase assays revealed that these miRNAs downregulate by directly interacting with the 3'-UTR of mRNA. Moreover, miR-135b-5p and miR-135a-5p suppress cell proliferation and migration in glioblastoma multiforme cells by inhibiting at the mRNA and protein levels. Taken together, our results provide novel regulatory mechanisms for at the post-transcriptional level and identify potential candidates for the treatment of patients with glioblastoma multiforme.