Broadband Microwave Electroporation Device for the Analysis of the Influence of Frequency, Temperature and Electrical Field Strength.
2022 44th Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC)(2022)
摘要
In this paper, a broadband microwave device for cell poration is presented, that enables the analysis of the relation between frequency, electrical field strengths and temperature for a successful cell poration. Electromagnetic-thermal coupled simulations in the frequency range from 1 GHz to 10 GHz show that the device reaches electrical field strengths of 100 V/cm and temperatures lower then 40°C. Electroporation experiments with adherent C2C12 mouse myoblast cells show successful uptake of an anti-histone γ -H2A.X nanobody at a frequency of 10 GHz. This MWP device allows the fast electro-poration of adherent cells. After 15 min, the cells show uptake of γ -H2A.X-specific nanobody while most of them survived.
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关键词
Animals,Electricity,Electroporation,Mice,Microwaves,Temperature
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