DNA Methylation Analysis of Cnr1 Gene Promoter.

Methods in molecular biology (Clifton, N.J.)（2023）

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摘要

DNA methylation pattern could be considered a biomarker to be exploited for the study and management of several human diseases. In this chapter, detailed protocols are provided for two experimental approaches used for quantitative methylation analysis of bisulfite converted DNA: methylation-specific PCR (MSP) and pyrosequencing.

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关键词

DNA extraction,DNA methylation,Methylation-specific PCR (MSP),Pyrosequencing,Type-1 cannabinoid receptor

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