Automated ssDNA SELEX Using Histidine-Tagged Target Proteins.
Methods in molecular biology (Clifton, N.J.)（2023）
摘要
The systematic evolution of ligands by exponential enrichment (SELEX) enables the identification of ssDNA or RNA sequences binding to different target molecules, highly specific and with high affinity. In this chapter, we describe a selection strategy with ssDNA for a histidine-tagged protein that could be either performed hands-on manually or fully automated by an appropriate robotic selection platform.
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关键词
Aptamer,Automated in vitro selection,Gel electrophoresis,Protein immobilization,SELEX,ssDNA
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