The Chick CAM as an In Vivo System to Study Stem Cell Activity.
Methods in molecular biology (Clifton, N.J.)(2023)
摘要
The chick embryo chorioallantoic membrane (CAM), an extensively vascularized extraembryonic membrane, has been widely used to study several aspects of tumor development including tumor-induced angiogenesis, tumor cell proliferation, and metastasis. Based on the tumor cell/CAM system, we focused here on the identification and quantification of cancer stem cells. We validated the CAM model as a suitable model to evaluate stem cell activity in a given mixed cell population.
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关键词
Cancer stem cells,Chorioallantoic membrane,Limiting dilution assays
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