PROMIS: Co-fractionation Mass Spectrometry for Analysis of Protein-Metabolite Interactions.
Methods in molecular biology (Clifton, N.J.)(2023)
摘要
The roles of small molecules in every aspect of life have been gaining increased recognition. Many are known to exert their effect by binding proteins-but a comprehensive overview of protein-metabolite interactions (PMIs) is missing. Recently we devised a non-targeted method for detecting PMIs using size-exclusion chromatography followed by proteomic and metabolomic analysis: PROMIS. Under test this method was able to identify known PMIs such as enzyme-cofactor complexes as well as novel ones.
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关键词
Co-fractionation,Ligand,Mass spectrometry,Protein,Protein–metabolite interactions,Size exclusion chromatography
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