Antibacterial activity of chitosan-polyethylene oxide nanofibers containing silver nanoparticles against aerobic and anaerobic bacteria

•The chemical reduction of chitosan and glucose as stabilizers led to the production of silver nanoparticles (SNPs).•A 30-minute sonication of the chitosan-silver nitrate solution was used to produce optimal silver nanoparticles.•This study assessed SNP/CS-PEO nanofibers for promising application in mixed anaerobic-aerobic infections.•SNP/CS-PEO nanofibers indicated favorable results in terms of mechanical and physicochemical characteristics.•MTT assay was performed to evaluate the biocompatibility of CS-PEO/SNP nanofibers against HDF and L929 cell lines.