Next generation VAMS®–Trypsin immobilization for instant proteolysis in bottom-up protein determination

•The tedious tryptic digestion step for proteomic analyses is incorporated in VAMS® samplers.•Smart proteolysis sampling initiates preparation already at sample collection.•Trypsin is covalently bound to VAMS® which than serves as smart sampler.•Smart sampler can be applied in a proteomic workflow.•Proteins in complex biological matrices like human serum are digested.