The Impact of Inoculation with Azotobacter and Azospirillum Bacteria and Chemical Fertilizer on Barley Growth (Hordeum Vulgare L.)

Abstract To investigate the effect of using bio-inoculation and chemical fertilizers on barley Hordeum vulgare L. growth, one experiment was applied using sterile soil with a silty clay loam at the Agriculture College Research Station, Tikrit University, Tikrit, Iraq. Completely Random Design (CRD) with two factors, bio and chemical fertilizers, and four levels for each was used to layout the experiment. The biofertilizers treatments were non as a check (NC), Azotobacter (AZ), Azospirillum (AS), Azotobacter and Azospirillum (ASAZ) while the chemical fertilizers levels were 0, 50, 75, and 100 % of barley fertilizers recommendation, which was 120 kg. h-1 NPK. the biofertilizers with ASAZ increased barley plant height, 33.28 cm, dry weight, 5.73 g. plant-1, root weight, 4.86 g. plant-1, and absorbent N, 2.97%, P, 0.35%, K, 2.61% for barley, which were higher than other treatments. Also, the results showed the interaction ASAZ with 75% of barley’s fertilizers recommendation had a higher average for all traits. In short, using biofertilizers reduced 25% of the chemical fertilizer cost.