Local Methods to Improve Cryo-Electron Microcopy Maps

S. Kaur,J. Gomez-Blanco,A. Khalifa,S. Adinarayanan,R. Sanchez-Garcia,D. Wrapp,J. S. McLellan,K. H. Bui,J. Vargas

OSA Imaging and Applied Optics Congress 2021 (3D, COSI, DH, ISA, pcAOP)（2021）

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摘要

Cryo-electron microscopy (cryo-EM) has become a powerful technique for protein structure determination. However, there are still important issues to be addressed, such as, robust sharpening or local B-factors estimations. To address these issues, we present here new local methods to improve the analysis and interpretability of cryo-EM maps.

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