microRNA 21 Promotes the Proliferation and Metastasis of Oral Squamous Cell Carcinoma by Targeting RECK
Journal of Hard Tissue Biology(2022)
摘要
We aimed to explore the effects of microRNA 21 (miR-21) downregulation on the expression of RECK and the proliferation and metastasis of oral squamous cell carcinoma (OSCC) cells. Sixty-five tumor tissue (TT) and corresponding paracancerous tissue (PT) specimens were excised from patients with OSCC. The mRNA levels of miR-21 and RECK in TTs and PTs were determined using qPCR. To assess the effects of miR-21-mediated modulation of RECK expression on tumor behavior, the OSCC cell line CAL-27 was transfected with miR-21 mimic, miR-21 inhibitor, or negative control (miR-NC). Compared with PTs, TTs showed increased levels of miR-21 and decreased mRNA and protein levels of RECK. Compared with CAL-27 cells transfected with miR-NC, those transfected with miR-21 inhibitor had upregulated RECK mRNA and protein expression, whereas those transfected with miR-21 mimic had downregulated RECK expression. After 48-72 h of transfection, the miR-21 mimic group exhibited higher cell proliferation rate than other groups. miR-21 was up-regulated and RECK expression was downregulated in OSCC. The downregulation of miR-21 upregulates RECK expres-sion via disinhibition, thereby suppressing the proliferation and migration and accelerating the apoptosis of OSCC cells. Thus, the downregulation of miR-21 may be an effective strategy against OSCC.
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关键词
Cal-27 cells, miR-21, Oral squamous cell carcinoma, RECK
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