Research Progress of The Peptide??N-Glycanase (PNGase) br

Peptide : N-glycanase(PNGase)is a deglycosylation enzyme widely presented in fungi,plants,mammals and other eukaryotics.Only two bacterial PNGase have being isolated(PNGase F and PNGase F-II)thusfar,and both are used widely as research tools in glycomics.PNGase catalyzed the hydrolysis of the amidebond between N-acetylglucosamine and an Asn residue on an N-glycoprotein,generating a de-N-glycosylatedprotein and a complete N-oligosaccharide.After the reaction,the N-glycosylated Asn residue was converted toAsp.Although it is known that PNGase participates in protein degradation,organ development,individual growthand other key biological processes in organisms,its impacts on health was illustrated only recently.HumanPNGase(NGLY1)deficiency could lead to a genetic disease named congenital disorder of deglycosylation-NGLY1.A nematode PNGase deficiency could reduce its life span.Its defects in mice could be embryonic lethal.This article describes the distribution,protein structure,and biological function of PNGase in different species.Itcan serve as an important information resource to support basic research for PNGase mechanism and innovativestudy for PNGase applications.