Lactiplantibacillus plantarum KAU007 Extract Modulates Critical Virulence Attributes and Biofilm Formation in Sinusitis Causing Streptococcus pyogenes .

is one of the most common bacteria causing sinusitis in children and adult patients. Probiotics are known to cause antagonistic effects on growth and biofilm formation. In the present study, we demonstrated the anti-biofilm and anti-virulence properties of KAU007 against ATCC 8668. The antibacterial potential of KAU007 metabolite extract (LME) purified from the cell-free supernatant of KAU007 was evaluated in terms of minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC). LME was further analyzed for its anti-biofilm potential using crystal violet assay and microscopic examination. Furthermore, the effect of LME was tested on the important virulence attributes of , such as secreted protease production, hemolysis, extracellular polymeric substance production, and cell surface hydrophobicity. Additionally, the impact of LME on the expression of genes associated with biofilm formation and virulence attributes was analyzed using qPCR. The results revealed that LME significantly inhibited the growth and survival of at a low concentration (MIC, 9.76 µg/mL; MBC, 39.06 µg/mL). Furthermore, LME inhibited biofilm formation and mitigated the production of extracellular polymeric substance at a concentration of 4.88 μg/mL in . The results obtained from qPCR and biochemical assays advocated that LME suppresses the expression of various critical virulence-associated genes, which correspondingly affect various pathogenicity markers and were responsible for the impairment of virulence and biofilm formation in . The non-hemolytic nature of LME and its anti-biofilm and anti-virulence properties against invoke further investigation to study the role of LME as an antibacterial agent to combat streptococcal infections.