Bacillus litorisediminis sp. nov., a Thermophilic Bacterium Isolated from Mangrove Sediment

Two aerobic, Gram-staining-positive, rod-shaped, endospore-forming, thermophilic bacterial strains, designated FJAT-47801 T and FJAT-47835, were isolated from the sediment collected from Zhangjiang Estuary Mangrove National Nature Reserve in Fujian Province, China. Growth was observed at 25–55 °C (optimum, 50 °C) and pH 7.0–9.0 (optimum, pH 7.0), with up to 4.0% (w/v) NaCl (optimum, without NaCl). Strains FJAT-47801 T and FJAT-47835 showed the highest 16S rRNA gene sequence similarity to Bacillus oleivorans (98.5%). The 16S rRNA gene sequence similarity between FJAT-47801 T and FJAT-47835 was 99.9% indicating they were the same species. Phylogenetic (based on 16S rRNA gene sequences) and phylogenomic (based on 120 conserved bacterial single-copy genes) trees showed that strains FJAT-47801 T and FJAT-47835 should be affiliated to the genus Bacillus . The of menaquinone of strain FJAT-47801 T was MK-7. The major fatty acids of strain FJAT-47801 T were iso-C 15:0 , anteiso-C 15:0 , iso-C 17:0 , and C 16:0 . The major polar lipids strain FJAT-47801 T were phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), and phosphatidylglycerol (PG). The genomic DNA G+C content of strain FJAT-47801 T was 39.3%. The average nucleotide identity (84.3%) and the digital DNA–DNA hybridization value (28.1%) between strain FJAT-47801 T and B. oleivorans CCTCC AB 2013353 T were below the cut-off level for species delineation. Based on the above results, strain FJAT-47801 T represents a novel species of the genus Bacillus , for which the name Bacillus litorisediminis sp. nov., is proposed. The type strain is FJAT-47801 T (=GDMCC 1.2712 T = JCM 34875 T ).