Optimized protocol for quantification of mitochondrial non-heme and heme iron content in mouse tissues and cultured cells.

STAR protocols(2023)

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摘要
Impaired mitochondrial iron metabolism is associated with aging and a variety of diseases, and there is a growing need to accurately quantify mitochondrial iron levels. This protocol provides an optimized method for evaluating non-heme and heme iron in mitochondrial and cytosolic fractions of tissues and cultured cells. Our protocol consists of three steps: sample fractionation, non-heme iron measurement, and heme iron measurement. For complete details on the use and execution of this protocol, please refer to Sato et al. (2022)..
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关键词
Cell Biology,Cell Separation/Fractionation,Metabolism,Molecular Biology
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