Photoactivated Localization Microscopy Combined with Image Spatial Correlation Analysis to Study of the Distribution and Diffusion of Angiotensin II Type 1 Receptor in the Plasma Membrane.

Super resolution fluorescence microscopy, (SRFM) techniques which overcome the spatial diffraction-limit has enabled us to visualize nanoscale cellular events with high temporal and spatial resolution in living cells, but in order to gain reliable mechanistic insights, a quantitative analysis is essential 1–3. In this study we used SRFM combined with novel image analysis as a powerful tool to investigate the mechanisms by which Angiotensin II type 1 Receptors (AT1Rs), are distributed and diffuse in the plasma membrane.