The biological effect of a beef-derived Latilactobacillus sakei on beef steaks during chilled storage.

The aim of this study was to investigate the biological inhibiting effect of a beef-derived (RS-25) on the spoilage of beef steaks in overwrapped packaging during the 12 days of storage at 4°C. Beef quality as well as microbial indicators were determined at different intervals during the storage after the inoculation of RS-25 at the 6 log CFU/g, and the high-throughput sequencing was applied to investigate the changes of microbial community structure during the storage. The inoculation of RS-25 on beef had no effect ( > .05) on pH, TBARS, and TVB-N during storage indicating the weak effect of such strain on the eat quality. Furthermore, the rise of and the delayed decline of and reveal the protection effect of RS-25 on the meat color. RS-25 reduced the re-contaminated by 1.16 log CFU/g ( < .01), and the growth of was also inhibited but no inhibition was found on the spp. at the first 6 days of storage. The inhibiting effect of RS-25 was covered by the rapid growth of other microorganism during the following 6 days of storage. Consistent with the microbial counts results, high-throughput sequencing analysis confirmed that the inoculated RS-25 was dominant at first 6 days, and then replaced by spp. The findings obtained from the current study may provide basic information for the further application of bioprotective bacteria in preservation of beef steaks in the overwrapped packaging.