Supplemental data from Tumor-Derived Osteopontin Suppresses Antitumor Immunity by Promoting Extramedullary Myelopoiesis

Supplemental data. Supplementary method, Supplementary figure legends and Supplementary figures 1-9. Supplementary figure 1. Gating strategies for the flow cytometric analysis. Supplementary figure 2. Changes of MDSCs, lymphoid cells and lymphoid progenitor cells in the CT26 TBM. Supplementary figure 3. Accumulated MDSCs in MC38 TBM. Supplementary figure 4. Increased osteopontin in MC38 TBM. Supplementary figure 5. In vivo proliferation and apoptosis of tumors. Supplementary figure 6. Changes of sub-populations of LK cells. Supplementary figure 7. Induction of extramedullary myelopoiesis by osteopontin from malignant tumor cells in the tumor microenvironment. Supplementary figure 8. The role of osteopontin in LK cell migration and MDSC survival. Supplementary figure 9. Osteopontin-induced LK cell proliferation was mediated by the CD44-ERK/MAPK signaling pathway.