In Situ Evaluation of Cellular Spheroid Stiffness by Pinching Device Composed of Piezoactuator and Strain Gauge

Mechanical characterization of cells is important for understanding their behavior. This paper reports on an in situ evaluation of the stiffness of cellular spheroids using a pinching device. The pinching device is integrated with a bimorph piezoactuator and strain gauge. It can pinch an object by combining a piezo bimorph actuator and a thin Si structure and can detect the reaction force from an elastic object using a strain gauge integrated into the thin Si structure. The strain gauge can detect the bending deformation of the device and estimate the reaction force by considering actuation. This paper presents the design of the pinching device and experimental results when used for the in situ evaluation of the stiffness of cellular spheroids. (c) 2023 Institute of Electrical Engineers of Japan. Published by Wiley Periodicals LLC.