Random matrix-based laser speckle contrast imaging enables quasi-3D blood flow imaging in laparoscopic surgery.

Laser speckle contrast imaging (LSCI) provides full-field and label-free imaging of blood flow and tissue perfusion. It has emerged in the clinical environment, including the surgical microscope and endoscope. Although traditional LSCI has been improved in resolution and SNR, there are still challenges in clinical translations. In this study, we applied a random matrix description for the statistical separation of single and multiple scattering components in LSCI using a dual-sensor laparoscopy. Both tissue phantom and rat experiments were performed to test the new laparoscopy in the laboratory environment. This random matrix-based LSCI (rmLSCI) provides the blood flow and tissue perfusion in superficial and deeper tissue respectively, which is particularly useful in intraoperative laparoscopic surgery. The new laparoscopy provides the rmLSCI contrast images and white light video monitoring simultaneously. Pre-clinical swine experiment was also performed to demonstrate the quasi-3D reconstruction of the rmLSCI method. The quasi-3D ability of the rmLSCI method shows more potential in other clinical diagnostics and therapies using gastroscopy, colonoscopy, surgical microscope, .