Antimicrobial Activity of Apis mellifera Bee Venom Collected in Northern Peru.

Due to the emergence of microorganisms resistant to antibiotics and the failure of antibiotic therapies, there is an urgent need to search for new therapeutic options, as well as new molecules with antimicrobial potential. The objective of the present study was to evaluate the in vitro antibacterial activity of venom collected in the beekeeping areas of the city of Lambayeque in northern Peru against , and . Bee venom extraction was performed by electrical impulses and separated using the Amicon ultra centrifugal filter. Subsequently, the fractions were quantified by spectrometric 280 nm and evaluated under denaturant conditions in SDS-PAGE. The fractions were pitted against ATCC 25922, ATCC 29213, and ATCC 27853. A purified fraction (PF) of the venom of and three low molecular weight bands of 7 KDa, 6 KDa, and 5 KDa were identified that showed activity against with a MIC of 6.88 µg/mL, while for and it did not present a MIC. No hemolytic activity at a concentration lower than 15.6 µg/mL and no antioxidant activity. The venom of A. mellifera contains a potential presence of peptides and a predilection of antibacterial activity against