Exploiting mosquito microbiome to combat the epidemic threat posed by Aedes aegypti

The mosquito larval gut is a major consortium for a wide array of microorganisms which has a direct impact on various host traits. The present study focus on the gut bacterial isolation from Aedes aegypti larvae. The phenotypic features of the isolate was elucidated by FE-SEM and identification was done by specific biochemical assay along with 16SrRNA sequencing. The evolutionary significance of bacteria was assessed by MEGA 11.0 software. To evaluate cross gut flora interaction we introduced gut bacteria belonging to common genera like Escherichia Coli , Salmonella typhi, Bacillus subtilis and Staphylococcus aureus to Aliiruegeria sabulilitoris along with broad spectrum antibiotic ampicillin as control in separate culture plates. The ammensalism was observed with Escherichia Coli and Salmonella typhi and stable co-existence with Bacillus subtilis and Staphylococcus aureus . For the first time from India, here we report a single bacterium Aliiruegeria sabulilitoris from Aedes aegypti larval gut. According to MEGA 11.0 software output the bacterium isolated in our study was closely related to samples isolated from neighbouring countries China and South Korea which strongly suggest a common ancestry. Generally larval gut harbour diversegroup of bacteria, here the presence of a single bacterium aggravate our curiosity to investigate future on this phenomenon. The bio-active compound present in the bacterium was found to be Diisobutyl Benzene-1, 2-Dicarboxylate and Tetrahydrodibenzothiophene which revealed by GC–MS technique. Collectively, these findings open a way towards exploiting the composition of mosquito gut flora in predicting the innovative concepts to combat the epidemic threats instigated by deadly diseases transmitted by the Aedes aegypti .