Batch and Continuous Flow Asymmetric Synthesis of Anabolic-Androgenic Steroids Via a Single-Cell Biocatalytic Δ1-dehydrogenation and C17β-carbonyl Reduction Cascade

Asymmetric synthesis of (+)-boldenone and (+)-boldenone undecylenate was achieved in batch and continuous flow, using an enzyme cascade process catalyzed by an engineered Δ1-KstD and a mined 17β-CR.