Tumor-derived microparticles-based nanomaterial as platform for delivery of tumor antigens to enhance immunogenicity

Background: Tumor-derived microparticles (TMPs) are not only carriers for tumor antigens but also can be used as good delivery platform. Therefore, for such an excellent delivery platform, we considered modifying TMPs with lipopolysaccharide (LPS) and polyethyleneimine (PEI) to synthesize TMPs-PEI-LPS for the delivery of tumor antigen and investigated its delivery efficiency, histocompatibility and anti-tumor effect. Result: We investigated the bioactivity of TMPs-PEI-LPS with dendritic cells and their potential for therapeutic application to treat lung cancer in mice models bearing Lewis lung carcinoma cells. Both in vitro and in vivo assays demonstrated successful TMPs-PEI-LPS induced the uptake and maturity of dendritic cells. TMPs-PEI-LPS exhibited certain immunostimulation activity and anti-tumor effects on inhibiting tumor growth in vivo. Notably, TMPs-PEI-LPS were safe and non-toxic in vivo study. Conclusion: These results indicated that TMPs-PEI-LPS are promising platforms for delivering tumor antigens to enhance immunogenicity.