Supplementary Figure S5 from Delivery of Therapeutics Targeting the mRNA-Binding Protein HuR Using 3DNA Nanocarriers Suppresses Ovarian Tumor Growth

Supplementary Fig. S5. A. 3DNA^® dendrimer platform for in vivo tumor-targeted delivery of siHuR. B. Ovarian cancer cell lines, non-tumorigenic human immortalized ovarian epithelial cells (HIO 80 and HIO 120), and murine ID8-Fluc cells express the folate receptor. C. Serum stability. siHuR incubated for 1 hr at 37^oC, then assayed on a 10%Tris/Borate/Urea PAGE. Sense strand: 42 bases (modified bases + extension); Antisense strand: 22 bases (modified bases). D. In vitro siHuR knockdown activity. siHuR (13nM) was combined with lipofectamine or targeted 3DNA dendrimer and incubated with A2780 cells overnight in 10% serum-containing medium. Dendrimer plus a scrambled siHuR had no knockdown activity (not shown).