Supplementary Figure 1. Ki67 staining and Western blots from Ceramide Kinase Promotes Tumor Cell Survival and Mammary Tumor Recurrence

(A) Quantification of MTB/TAN primary tumor cell lines expressing pk1, Cerk or G198D- Cerk withdrawn from doxycycline 72 hr then stained for Ki67. (B) Quantification of MTB/TAN primary tumor cell lines expressing MLP, a control scrambled shRNA, shCerk1 or shCerk2 withdrawn from doxycycline 72 hr then stained for Ki67. (C) Western blot of MTB/TAN primary tumor cell lines expressing empty vector, a control scrambled shRNA, or shCerk1 withdrawn from doxycycline 48 hr, blotted for cleaved PARP, quantified in (D), and cleaved caspase-3, quantified in (E).