Supplementary Figure S2 from <i>Ex Vivo</i> Expansion of Highly Cytotoxic Human NK Cells by Cocultivation with Irradiated Tumor Cells for Adoptive Immunotherapy

Supplementary Figure S2 PDF file - 39K, A standard 51Cr release assay was performed using K562, U937 or KL-1 as target cell lines, as described in the "Materials and Methods" section. These targets were labeled with 51Cr (Perkin Elmer, Boston, MA, USA) at 50 ��Ci/5 �~ 105 cells and incubated at 5 �~ 103 cells/well with resting human NK cells at E:T ratios of 30:1, 10:1 and 3:1. Percent specific lysis was calculated as follows: 100 �~ (experimental release-spontaneous release)/(maximum release-spontaneous release)