Supplementary Figure 1 from Clusterin Mediates TGF-β–Induced Epithelial–Mesenchymal Transition and Metastasis via Twist1 in Prostate Cancer Cells

PDF file - 90K, Slug and Snail don't affect CLU expression. A. PC-3 cells were transfected with 40 nmol/L of the indicated siRNA. At 72 h after transfection, quantitative RT-PCR was performed using the primers and probes for CLU and GAPDH. Each transcript level from cells transfected with control siRNA was set as 1. Boxes, mean; bars, �s.d. B. PC-3 cells were transfected with 40 nmol/L of the indicated siRNA. At 72 h after transfection, whole-cell extracts were analyzed by SDS-PAGE and western blot analysis with specific antibodies