Supplementary Figure 1 from Molecular Modulation of Estrogen-Induced Apoptosis by Synthetic Progestins in Hormone Replacement Therapy: An Insight into the Women's Health Initiative Study

Supplementary Figure 1. A. MCF-7:WS8 and MCF-7:5C cells were treated with either vehicle for 72 hours or 1nM E2 for 24 or 72 hours. Cells were then harvested, and ERα, PR, and GR proteins were measured by Western blot. MCF-7:WS8 cells were used as a positive control for PR expression. B. MCF-7:5C cells were treated with vehicle, 1nM E2, 1µM Dex, MPA, or NETA, or combinations of 1nM E2 plus 1µM Dex, MPA, or NETA for two months. Cells were harvested, proteins were extracted, and Western blots for ERα and GR protein levels were performed. β-actin was used as a loading control.