Supplementary Figures S1-S7 from Novel Cell-Penetrating Peptide-Based Vaccine Induces Robust CD4<sup>+</sup> and CD8<sup>+</sup> T Cell–Mediated Antitumor Immunity

Peptides and proteins used in the study, with all the minimal epitopes including 4 flanking residues (S1); Purified proteins were analyzed by Coomassie blue and Western Blot (S2); Z12 transports efficiently its cargo into the cytoplasm of DC (S3); Choice of adjuvant influences proportions of effector and memory phenotype antigen-specific CD8+ T cells induced after Z12-OVALP vaccination (S4); Persistance of antigen-specific cytotoxic T cells after vaccination (S5); Balance of brain tumor infiltrating lymphocytes is altered by Z12-OVALP vaccination (S6); Effector cytokine expression by brain tumor infiltrating CD8+ T cells is augmented by Z13-MultiE2 vaccination (S7).