Data Supplement from Molecular Homology and Difference between Spontaneous Canine Mammary Cancer and Human Breast Cancer

Fig. S1. Supplementary data for large scale genomic aberrations illustrated in Figure 2, including: A) the distribution of mapped read density of WGS; B) PCR confirmation of translocation and fusion junctions of a superamplicon; and C) PCR and RT-PCR amplification of MGAM, ZFAND3, and the ZFAND3-MGAM fusion gene. Fig. S2. The distribution of mapped read density of WES, supplementary data for Figure 3. Fig. S3. Differentially expressed genes and functions among complex, simple carcinomas and normal mammary glands, supplementary data for Figure 3. Fig. S4. IHC images of histone modifications in canine normal mammary glands at 400X magnification, supplementary data for Figure 4. Fig. S5. IHC images of histone modifications in simple carcinomas, complex carcinomas and normal mammary glands at 200X magnification, supplementary data for Figure 5. Fig. S6. Supplementary data for homology between canine MCs and human breast cancers described in Figure 6. These include differentially expressed genes between ER+ and ER- carcinomas at FDR ≤ 0.1 and an example of PAM50 clustering indicating canine and human tumor IDs.