Supplementary Data from Constant Degradation of the Androgen Receptor by MDM2 Conserves Prostate Cancer Stem Cell Integrity

The file contains 6 supplementary figures. Figure S1 shows that AR protein expression is also induced following treatment with additional proteasomal inhibitors, MG115 and Epoxomicine. Figure S2 compares the levels of MDM2 expression, demonstrating that CSC-like HuSLC and HPET cells express higher levels of MDM2 as compared to standard PCa cell lines. Figure S3 demonstrates that HPET and HuSLCs only express AR-fl mRNA but not AR-V mRNA and this is confirmed in Figure S4 where HPET and HuSLCs only express AR-fl protein but not AR-V protein. Figure S5 provides information that induction of AR promotes expression of additional AR target genes, namely AR-regulated HES1 and HEY1 genes. Figure S6 compares p53 expression in CSC-like HPET and HuSLCs to that of LNCaP cells.