Supplemental Materials and Methods from Combined Inhibition of DNMT and HDAC Blocks the Tumorigenicity of Cancer Stem-like Cells and Attenuates Mammary Tumor Growth

Rajneesh Pathania,Sabarish Ramachandran,Gurusamy Mariappan,Priyanka Thakur,Huidong Shi,Jeong-Hyeon Choi,Santhakumar Manicassamy,Ravindra Kolhe,Puttur D. Prasad,Suash Sharma,Bal L. Lokeshwar,Vadivel Ganapathy,Muthusamy Thangaraju

crossref（2023）

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摘要

The Supplemental materials and method file has methods for the Generation of mammospheres and tumorospheres, Preparation of single cell suspension from mammary gland, methods for RNA Isolation and Real-time PCR, and Clonogenic assay.

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