Double pulse irradiation of fs laser for enhancing the performance of precise laser sorting method

Cell sorting systems are necessary for large amount cell analysis in biological, medical, and clinical applications. As one of the highest throughput cell sorting technologies (100 kilohertz (kHz)), femtosecond (fs) laser-based cell manipulation systems have the potential for single-step multi-sorting. To further improve the performance, instead of single pulse irradiation mode of laser, double pulses irradiation mode was developed. In this research, after experimental investigations and numerical analysis, we found that a pulse interval of 10 mu s could achieve larger displacement of the sample than single ones.