dU-Tn5 stranded RNA-seq experiment v1
crossref（2022）
摘要
In this protocol, we describe a novel Tn5-based stranded RNA-seq procedure, in which deoxy-UTP-labeled Tn5 (dU-Tn5) is applied in library construction to preserve the strand-specificity of transcripts. The stranded library preparation section only contains 8 steps, which is straightforward and easy-to-do for library preparation.
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