The E3 ubiquitin ligase TRIM9 ubiquitin-modified Beclin1 regulates autophagy to relieve myocardial ischemia reperfusion injury

Research Square (Research Square)(2022)

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摘要
Abstract TRI partite motif (TRIM) family proteins are RING finger domain-containing proteins that are related to the maintenance of cellular functions. TRIM9, a pivotal E3 ubiquitin ligase, and a member of TRIM family, has been implicated in the pathogenesis of many diseases. However, the role of TRIM9 in myocardial ischemia–reperfusion injury (MIRI) remains unknown. In this study, we found that TRIM9 expression is upregulated in MIRI in vivo and in vitro. The inhibition of TRIM9 expression further increased the protein levels of LC3II and p62, and they were elevated in the process of MIRI, suggesting the disruption of autophagic flow. Furthermore, we examined the cell viability, the result showed that when the ischemia reperfusion and TRIM9 knocked down both happened, which harmful to H9c2. Mechanistically, TRIM9 downregulated the expression of Beclin1, a crucial protein in autophagy, and promoted the ubiquitination of Beclin1, followed by a decreased expression of LC3II and p62. Collectively, our data revealed that TRIM9 could protect against MIRI and might be a novel influence factor for cardiovascular disease.
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关键词
e3 ubiquitin,myocardial ischemia reperfusion injury,autophagy,beclin1,myocardial ischemia,ubiquitin-modified
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