High-throughput RNA isoform sequencing using programmed cDNA concatenation

Nature Biotechnology(2023)

Cited 0|Views4
No score
Full-length RNA-sequencing methods using long-read technologies can capture complete transcript isoforms, but their throughput is limited. We introduce multiplexed arrays isoform sequencing (MAS-ISO-seq), a technique for programmably concatenating complementary DNAs (cDNAs) into molecules optimal for long-read sequencing, increasing the throughput >15-fold to nearly 40 million cDNA reads per run on the Sequel IIe sequencer. When applied to single-cell RNA sequencing of tumor-infiltrating T cells, MAS-ISO-seq demonstrated a 12- to 32-fold increase in the discovery of differentially spliced genes.
Translated text
Key words
Gene expression analysis,RNA sequencing,RNA splicing,Life Sciences,general,Biotechnology,Biomedicine,Agriculture,Biomedical Engineering/Biotechnology,Bioinformatics
AI Read Science
Must-Reading Tree
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined