Ad hoc laboratory-based surveillance of SARS-CoV-2 by real-time RT-PCR using minipools of RNA prepared from routine respiratory samples

Anna M. Eis-Hübinger,Mario Hönemann,Jürgen J. Wenzel,Annemarie Berger,Marek Widra,Barbara Schmidt,Souhaib Aldabbagh,Benjamin Marx,Hendrik Streeck,Sandra Ciesek,Uwe. G. Liebert,Daniela Huzly,Hartmut Hengel,Marcus Panning

medRxiv (Cold Spring Harbor Laboratory)（2020）

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摘要

AbstractWe report a laboratory-based surveillance for SARS-CoV-2 using minipools of respiratory samples submitted for routine diagnostics. We tested a total of 70 minipools resembling 700 samples shortly before the upsurge of cases in Germany. We identified one SARS-CoV-2 positive patient. Our approach proved its concept, is easily adaptable and resource-saving.

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关键词

rna,respiratory,laboratory-based,sars-cov,real-time,rt-pcr

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