Peroxidases from grass clippings for the removal of phenolic compounds from wastewater

Green waste, especially of municipal origin, is currently used as a material only to a limited extent. However, the large material flows could also be used in a more economical way if they were integrated into biorefinery concepts. Besides the production of basic and fine chemicals, green waste could also be used as source of industrial relevant enzymes. Here, the purification and characterization of peroxidases from common lawn grass species Lolium perenne and Festuca arundinacea are reported. The purified peroxidase fractions as well as crude extracts were investigated for the removal of common wastewater pollutants such as phenol, m-cresol, and 2,4dichlorophenol by oxidative polymerization. The grass-derived peroxidases showed the highest affinity towards 2,4-dichlorophenol, followed by m-cresol and phenol. A crude extract of real lawn grass was able to remove over 95 % of 0.5 mM 2,4-dichlorophenol within 20 min.