A FAP-targeting minibody-IRDye700DX for photodynamic therapy of the cancer associated fibroblast

The cancer associated fibroblast (CAF) orchestrates an aberrant tumor microenvironment in pancreatic ductal adenocarcinoma (PDAC), contributing to poor patient survival. Fibroblast activation protein (FAP)-targeted depletion of the CAF in PDAC could modulate the tumor microenvironment and make it more susceptible to immune response or systemic therapies. Here, we developed a FAP-targeting minibody conjugated to the photosensitizer IRDye700DX for ablation of the CAF with photodynamic therapy. We characterized efficiency of the minibody-IRDye700X in vitro and in vivo in a murine subcutaneous PDAC model.