Proteomic identification of metabolic changes in Paracoccidioides brasiliensis induced by a nitroheteroarylchalcone.

To access the metabolic changes caused by a chalcone derivative () through a proteomic approach. Proteomic analysis was performed after 9 h of yeast () cell incubation with the at MIC. The proteomic findings were validated through and assays. Exposure to the compound led to the downregulation of proteins associated with glycolysis and gluconeogenesis, β-oxidation, the citrate cycle and the electron transport chain. caused an energetic imbalance in the fungus metabolism and deep oxidative stress. Additionally, the molecular docking approach pointed to this molecule as a putative competitive inhibitor of DHPS.